| ncbi-epcr | Tool to test a DNA sequence for the presence of sequence tagged sites | Mehr ... |
Electronic PCR (e-PCR) is computational procedure that is used to identify
sequence tagged sites(STSs), within DNA sequences. e-PCR looks for potential
STSs in DNA sequences by searching for subsequences that closely match the
PCR primers and have the correct order, orientation, and spacing that could
represent the PCR primers used to generate known STSs.
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The new version of e-PCR implements a fuzzy matching strategy. To reduce
likelihood that a true STS will be missed due to mismatches, multiple
discontigous words may be used instead of a single exact word. Each of this
word has groups of significant positions separated by 'wildcard' positions
that are not required to match. In addition, it is also possible to allow
gaps in the primer alignments.
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The main motivation for implementing reverse searching (called Reverse e-PCR)
was to make it feasible to search the human genome sequence and other large
genomes. The new version of e-PCR provides a search mode using a query
sequence against a sequence database. |
| ncbi-epcr-data | [Biology] NCBI public STS data | Mehr ... |
