| primer3 | Tool to design flanking oligo nucleotides for DNA amplification | Mehr ... |
Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as
criteria oligonucleotide melting temperature, size, GC content and
primer-dimer possibilities, PCR product size, positional constraints within
the source sequence, and miscellaneous other constraints. All of these
criteria are user-specifiable as constraints, and some are specifiable as
terms in an objective function that characterizes an optimal primer pair.
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It has been published in Rozen S and Skaletsky H, "Primer3 on the WWW for
general users and for biologist programmers.", Methods Mol Biol.
2000;132:365-86.
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The Whitehead Institute for Biomedical Research provides a web-based
front end to Primer3. |
